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tcf/lef reporter gene topflash (optimal tcf binding site)  (Upstate Biotechnology Inc)

 
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    Structured Review

    Upstate Biotechnology Inc tcf/lef reporter gene topflash (optimal tcf binding site)
    Tcf/Lef Reporter Gene Topflash (Optimal Tcf Binding Site), supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reporter+gene+(topflash%3B+optimal+lef-binding+site)/topflash+reporter+plasmid/pmc06162946-46-31-39
    Average 90 stars, based on 1 article reviews
    tcf/lef reporter gene topflash (optimal tcf binding site) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Opposing Roles of WNT-5A and WNT-11 in Interleukin-1β Regulation of Type II Collagen Expression in Articular Chondrocytes
    Article Snippet: .. Briefly, chondrocytes were transfected with 1 g of reporter gene (TOPFLASH; optimal Lef-binding site) or control gene (FOPFLASH;mutatedLef-bindingsite) (UpstateBiotechnology, Lake Placid, NY) and 1 g of pCMV- -galactosidase using Metafectene. ..

    Control:

    Article Title: Opposing Roles of WNT-5A and WNT-11 in Interleukin-1β Regulation of Type II Collagen Expression in Articular Chondrocytes
    Article Snippet: .. Briefly, chondrocytes were transfected with 1 g of reporter gene (TOPFLASH; optimal Lef-binding site) or control gene (FOPFLASH;mutatedLef-bindingsite) (UpstateBiotechnology, Lake Placid, NY) and 1 g of pCMV- -galactosidase using Metafectene. ..

    other:

    Article Title: Response to tumor necrosis factor-α mediated inflammation involving activation of prostaglandin E2 and Wnt signaling in nucleus pulposus cells.
    Article Snippet: To examine b-catenin–T cell factor (TCF)/lymphoid enhancer-binding factor (LEF) transcriptional activity, NP and AF cells were transiently transfected with the TCF/LEF reporter gene Topflash (optimal TCF binding site) or Fopflash (mutated TCF binding site) (Upstate Biotechnology, Lake Placid, NY).

    Article Title: The relationship between the Wnt/β-catenin and TGF-β/BMP signals in the intervertebral disc cell.
    Article Snippet: Wnt/b-Catenin and TGF-b/BMP Signals in the Intervertebral Disc Cell AKIHIKO HIYAMA,* DAISUKE SAKAI, MASAHIRO TANAKA, FUMIYUKI ARAI, DAISUKE NAKAJIMA, KOICHIRO ABE, AND JOJI MOCHIDA Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, Isehara, Kanagawa, Japan Research Center for Regenerative Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine,

    Article Title: A complex interaction between Wnt signaling and TNF-α in nucleus pulposus cells
    Article Snippet: To determine the β-catenin-TCF/LEF transcriptional activity, nucleus pulposus cells and annulus fibrosus cells were transiently transfected with the TCF/LEF reporter gene Topflash (optimal TCF binding site) or Fopflash (mutated TCF binding site) (Upstate Biotechnology, Lake Placid, NY, USA).

    Article Title: CCN family member 2/connective tissue growth factor (CCN2/CTGF) is regulated by Wnt–β-catenin signaling in nucleus pulposus cells
    Article Snippet: To determine the β-catenin–T cell factor (TCF)/lymphoid enhancing factor (LEF) transcription activity after treatment with 6-bromoindirubin-3′-oxime (BIO) (number 361550; Calbiochem, San Diego, CA, USA), NP cells were transiently transfected with the TCF/LEF reporter gene Topflash (optimal TCF binding site) (Upstate Biotechnology).



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    Upstate Biotechnology Inc tcf/lef reporter gene topflash (optimal tcf binding site)
    Tcf/Lef Reporter Gene Topflash (Optimal Tcf Binding Site), supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reporter+gene+(topflash%3B+optimal+lef-binding+site)/topflash+reporter+plasmid/pmc06162946-46-31-39
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